Efficient cell-wide mapping of mitochondria in electron microscopic volumes using webKnossos.

ABSTRACT: Recent technical advances in volume electron microscopy (vEM) and artificial-intelligence-assisted image processing have facilitated high-throughput quantifications of cellular structures, such as mitochondria, that are ubiquitous and morphologically diversified. A still often-overlooked computational challenge is to assign a cell identity to numerous mitochondrial instances, for which both mitochondrial and cell membrane contouring used to be required. Here, we present a vEM reconstruction procedure (called mito-SegEM) that utilizes virtual-path-based annotation to assign automatically segmented mitochondrial instances at the cellular scale, therefore bypassing the requirement of membrane contouring. The embedded toolset in webKnossos (an open-source online annotation platform) is optimiz

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC11955265 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature