Fluorescent Reporter-Based Fiber Optic Probe for Continuous Detection of Antibodies.

ABSTRACT: Measurement of antibody and antibody fusion protein concentration is vital for process development and manufacturing. Continuous, in-line monitoring of antibody concentration could be useful in a variety of applications, such as controlling the loading of protein A columns to prevent breakthrough, monitoring bioreactor titer, and detecting leaks past ultrafiltration/diafiltration membranes. Molecule-specific monitoring techniques are advantageous for antibody detection in cell culture fluid in the presence of complex process impurities. Here we report a continuous in-line, real-time IgG monitoring platform using a fiber-optic biosensor with a replaceable sensor tip covalently functionalized with a fluor-labeled protein consisting of a pentamer of the Z domain (a more stable form of the B d

SUBMITTER: Nandy S 

PROVIDER: S-EPMC11955272 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature