A GTP-driven central carbon metabolism in the cellulolytic bacterium Ruminiclostridium cellulolyticum.

ABSTRACT: In Ruminiclostridium cellulolyticum, the hexokinase and galactokinase were formerly shown to strongly prefer GTP over ATP, whereas the phosphofructokinase is PPi-dependent, suggesting an unconventional central carbon metabolism in this anaerobic bacterium. Herein, the characterization of all other kinases of this pivotal pathway led to the identification of their preferred NTP/NDP. The kinases involved in the first reactions, primarily functioning as NTP-consuming enzymes, appear to be GTP-dependent. In contrast, the enzymes catalyzing the downstream steps that mainly generate NTP, show no marked preference. Consequently, its central carbon metabolism appears essentially driven by GTP, whose cellular content nears that of ATP. Interestingly, in vivo reciprocal exchange of the GTP-dependent

SUBMITTER: Liu N 

PROVIDER: S-EPMC11955521 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature