Using short-read 16S rRNA sequencing of multiple variable regions to generate high-quality results to a species level.

ABSTRACT:

Introduction

Short-read amplicon sequencing studies have typically focused on 1-2 variable regions of the 16S rRNA gene. Species-level resolution is limited in these studies, as each variable region enables the characterisation of a different subsection of the microbiome. Although long-read sequencing techniques can take advantage of all 9 variable regions by sequencing the entire 16S rRNA gene, short-read sequencing has remained a commonly used approach in 16S rRNA research. This work assessed the feasibility of accurate species-level resolution and reproducibility using a relatively new sequencing kit and bioinformatics pipeline developed for short-read sequencing of multiple variable regions of the 16S rRNA gene. In addition, we evaluated the potential impact of different sample

SUBMITTER: Graham AS 

PROVIDER: S-EPMC11955629 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature