Single-nuclei transcriptome analysis of IgM⁺ cells isolated from channel catfish (<i>Ictalurus punctatus</i>) spleen.

ABSTRACT: Catfish production is the primary aquaculture sector in the United States, and the key cultured species is channel catfish (Ictalurus punctatus). The major causes of production losses are pathogenic diseases, and the spleen, an important site of adaptive immunity, is implicated in these diseases. To examine the channel catfish immune system, single-nuclei transcriptomes of sorted and captured IgM⁺ cells were produced from adult channel catfish. Three channel catfish (~1 kg) were euthanized, the spleen dissected, and the tissue dissociated. The lymphocytes were isolated using a Ficoll gradient and IgM⁺ cells were then sorted with flow cytometry. The IgM⁺ cells were lysed and single-nuclei libraries generated using a Chromium Next GEM Single Cell 3' GE

SUBMITTER: Aldersey JE 

PROVIDER: S-EPMC11955638 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature