Jinxin oral liquid reduced lung inflammation in influenza A virus infected mice through inhibiting NOD-like receptor protein 3 pathway.

ABSTRACT:

Objective

To investigate the therapeutic effects of Jinxin oral liquid (, JX) on influenza A virus（H1N1）influenza virus infected mice.

Methods

We established a model of by intranasally infecting the mice with H1N1 virus. The mice were then orally administered JX or ribavirin to evaluate their therapeutic effects in vivo. We conducted histologic and immunohistochemical analyses, enzyme linked immunosorbent assay or quantitative real-time polymerase chain reaction to assess lung damage and the expression of inflammatory cytokines. Western blot (WB) experiments was conducted to measure the activation of NOD-like receptor protein 3 (NLRP3) pathway. Flow cytometry was employed to quantify the populations of alveolar macrophages (AMs). To block the NLRP3 pathway, mice were treated

SUBMITTER: Tao LI 

PROVIDER: S-EPMC11955749 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature