From Bioreactor to Bulk Rheology: Achieving Scalable Production of Highly Concentrated Circular DNA.

ABSTRACT: DNA serves as a model system in polymer physics due to its ability to be obtained as a uniform polymer with controllable topology and nonequilibrium behavior. Currently, a major obstacle in the widespread adoption of DNA is obtaining it on a scale and cost basis that accommodates bulk rheology and high-throughput screening. To address this, recent advancements in bioreactor-based plasmid DNA production is coupled with anion exchange chromatography producing a unified approach to generating gram-scale quantities of monodisperse DNA. With this method, 1.1 grams of DNA is obtained per batch to generate solutions with concentrations up to 116 mg mL^-1. This solution of uniform supercoiled and relaxed circular plasmid DNA, is roughly 69 times greater than the overlap concentration. Th

SUBMITTER: Paiva WA 

PROVIDER: S-EPMC11955860 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature