Effects of the <i>skp1</i> gene of the SCF complex on lipid metabolism and response to abiotic stress in <i>Chlamydomonas reinhardtii</i>.

ABSTRACT: SKP1 (S-phase kinase-associated protein 1) is a key member of the SCF (SKP1-Cullin1-F-box) E3 ligase complex. The SCF complex is involved in regulating various levels of plant physiology, including regulation of cellular signaling and response to abiotic stresses. While the function of SKP1 in plants is well known, its function in algae remains poorly understood. In this study, we investigated the role of the Chlamydomonas reinhardtii skp1 gene using RNAi interference and overexpression approaches. Subcellular localization of SKP1 was performed by transient expression in onion epidermal cells. For abiotic stress assays, the growth of skp1 RNAi and overexpression recombinant strains was examined under conditions of high osmolality (sorbitol), high salinity (NaCl) and high temp

SUBMITTER: Deng XD 

PROVIDER: S-EPMC11955966 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature