Engineering CO₂-fixing modules in Escherichia coli via efficient assembly of cyanobacterial Rubisco and carboxysomes.

ABSTRACT: Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) is the central enzyme for conversion of atmospheric CO₂ into organic molecules, playing a crucial role in the global carbon cycle. In cyanobacteria and some chemoautotrophs, Rubisco complexes, together with carbonic anhydrase, are enclosed within specific proteinaceous microcompartments known as carboxysomes. The polyhedral carboxysome shell ensures the dense packaging of Rubisco and creates a high-CO₂ internal environment to facilitate CO₂ fixation. Rubisco and carboxysomes have been popular targets for bioengineering, with the intent of enhancing plant photosynthesis, crop yields, and biofuel production. However, efficient generation of Form 1B Rubisco and cyanobacterial β-carboxysomes in heterolog

SUBMITTER: Sun Y 

PROVIDER: S-EPMC11956089 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature