Lung allograft dysbiosis associates with immune response and primary graft dysfunction.

ABSTRACT:

Background

Lower airway enrichment with oral commensals has been previously associated with severe primary graft dysfunction (PGD) after lung transplantation (LT). We aimed to determine whether this dysbiotic signature is present across all PGD severity grades and whether it is associated with a distinct host inflammatory endotype.

Methods

Lower airway samples from 96 LT recipients were used to evaluate the lung allograft microbiota via 16S rRNA gene sequencing. Bronchoalveolar lavage (BAL) cytokine concentrations and cell differential percentages were compared across PGD grades. In a subset of samples, we evaluated the lower airway host transcriptome using RNA sequencing methods.

Results

Differential analyses demonstrated lower airway enrichment with supraglottic-pre

SUBMITTER: Nelson NC 

PROVIDER: S-EPMC11956144 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature