Vitreous Fibrillar Structure and Interfibrillar Composition in Adult Mice.

ABSTRACT:

Purpose

Although mice are widely used models for human eye diseases, knowledge of their vitreous is scarce and fragmentary. This study characterizes the structure and composition of vitreous fibrils and their interfibrillar space. Given the role of this fibrillar network in maintaining the vitreous' biomechanical properties, the impact of intravitreal injections on vitreous architecture was also explored.

Methods

Healthy adult C57/BL6J mice were studied. Classical histological techniques, picrosirius red-polarization, immunofluorescence, lectin histochemistry, and hyaluronic acid binding protein assays were used to examine vitreous fibrils and interfibrillar space. For the analysis of distribution of components along the fibrils, the Airyscan microscopy was used to achieve h

SUBMITTER: Jaramillo-Garces P 

PROVIDER: S-EPMC11956745 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature