Noise-guided tuning of synthetic protein waves in living cells.

ABSTRACT: Biological systems use protein circuits to organize cellular activities in space and time, but engineering synthetic dynamics is challenging due to stochastic effects of genetic and biochemical variation on circuit behavior. Genetically encoded oscillators (GEOs) built from bacterial MinDE-family ATPase and Activator modules generate fast orthogonal protein waves in eukaryotic cells, providing an experimental model system for genetic and biochemical coordination of synthetic protein dynamics. Here, we use budding yeast to experimentally define and model phase portraits that reveal how the breadth of frequencies and amplitudes available to a GEO are genetically controlled by ATPase and Activator expression levels and noise. GEO amplitude is encoded by ATPase absolute abundance, making it se

SUBMITTER: Bolshakov DT 

PROVIDER: S-EPMC11957142 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature