Single-nucleosome mapping of histone modifications in S. cerevisiae.

ABSTRACT: Covalent modification of histone proteins plays a role in virtually every process on eukaryotic DNA, from transcription to DNA repair. Many different residues can be covalently modified, and it has been suggested that these modifications occur in a great number of independent, meaningful combinations. Published low-resolution microarray studies on the combinatorial complexity of histone modification patterns suffer from confounding effects caused by the averaging of modification levels over multiple nucleosomes. To overcome this problem, we used a high-resolution tiled microarray with single-nucleosome resolution to investigate the occurrence of combinations of 12 histone modifications on thousands of nucleosomes in actively growing S. cerevisiae. We found that histone modifications do not

SUBMITTER: Liu CL 

PROVIDER: S-EPMC1195719 | biostudies-literature | 2005 Oct

REPOSITORIES: biostudies-literature