Dynamic imaging of myelin pathology in physiologically preserved human brain tissue using third harmonic generation microscopy.

ABSTRACT: Myelin pathology is known to play a central role in disorders such as multiple sclerosis (MS) among others. Despite this, the pathological mechanisms underlying these conditions are often difficult to unravel. Conventional techniques like immunohistochemistry or dye-based approaches, do not provide a temporal characterization of the pathophysiological aberrations responsible for myelin changes in human specimens. Here, to circumvent this curb, we present a label-free, live-cell imaging approach of myelin using recent advancements in nonlinear harmonic generation microscopy applied to physiologically viable human brain tissue from post-mortem donors. Gray and white matter brain tissue from epilepsy surgery and post-mortem donors was excised. To sustain viability of the specimens for several

SUBMITTER: Meijns NRC 

PROVIDER: S-EPMC11957345 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature