Alteration in ATR protein level does not account for the inherent radiosensitivity of HPV-positive head and neck squamous cell carcinoma.

ABSTRACT:

Objectives

Human papilloma virus (HPV)-positive head and neck squamous cell carcinoma (HNSCC) cells are highly radiosensitive resulting from an elevated number of DNA double-strand breaks (DSB) remaining after irradiation. Partially this effect is due to a defective homologous recombination (HR). HPV-positive cells also show pronounced instability of chromosome 3, which codes for the kinase ataxia-telangiectasia and Rad3-related (ATR) protein, a central player of HR. If there is a contribution of ATR to the radiosensitivity of HPV-positive cells remains unclear, and this in-vitro study tested a functional involvement of ATR expression.

Methods

The study was performed with six HPV-negative and six HPV-positive HNSCC cell lines. Gene copy number and gene expression were determ

SUBMITTER: Kohl S 

PROVIDER: S-EPMC11957528 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature