Spatial analysis of airborne bacterial concentrations and microbial communities in a large-scale commercial layer facility.

ABSTRACT: This study investigated the spatial distribution patterns of airborne bacterial concentrations and microbial community structures in a modern commercial layer facility housing approximately 50,000 laying hens equipped with advanced environmental control systems. Air samples were systematically collected at 50 strategically distributed locations using a six-stage Andersen microbial air sampler, while environmental samples (dust, manure, intestinal contents) were characterized using 16S rRNA gene sequencing. Results demonstrated a distinct longitudinal gradient in airborne bacterial concentrations, progressively increasing from the air inlet (883±177 CFU/m³) to exhaust fans (12,650±813 CFU/m³), with a facility-wide mean concentration of 5,618±530 CFU/m³. Spatial analysis revealed significant

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC11957673 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature