Project description:HMEC240L cells were grown in standard 8 well MEMA for 72h and then fixed using 2% paraformaldehyde. Cells were stained with DAPI (nuclei), KRT5(basal lineage), KRT19(luminal lineage) and EdU(Active S phase). The cells were imaged on a Nikon HCA microscopy system, segmented with Cell Profiler, normalized using RUV and lowess using the spatial residuals as controls, and analyzed to identify microenvironment conditions that altered phenotypes of interest.

2016-11-10 | LDS-1439 | LINCS

MGH (CMT) Growth Inhibition Assay Protocol (9 compound doses: 0.512 uM to 0.002 uM) -- DNA Staining

Project description: Not available

2014-05-30 | EDS-1007 | LINCS

MGH (CMT) Growth Inhibition Assay Protocol (9 compound doses: 10.24 uM to 0.04 uM) -- DNA Staining

Project description: Not available

2014-05-30 | EDS-1010 | LINCS

MEMA PC3 Staining Set 1

Project description:Cell cycle and nuclear state imaging readouts for PC3 cells treated with ligand and ECMp combinations to explore the role of microenvironmental signals in cancer growth.

2016-03-09 | LDS-1311 | LINCS

MEMA PC3 Staining Set 2

Project description:Cell cycle and nuclear state imaging readouts for PC3 cells treated with ligand and ECMp combinations to explore the role of microenvironmental signals in cancer growth.

2016-03-09 | LDS-1315 | LINCS

MGH (CMT) Growth Inhibition Assay Protocol (9 compound doses: 64 nM to 0.25 nM) -- DNA Staining

Project description: Not available

2014-05-30 | EDS-1009 | LINCS

MEMA AU565 Staining Set 100 (Lapatinib treatment)

Project description:AU565 cells were grown in media with Lapatinib in 8 well MEMA for 72h and then fixed using 2% paraformaldehyde. Cells were stained with DAPI (nuclei), KRT5(Basal lineage), KRT19 (luminal lineage) and EdU (S phase activity). The cells were imaged on a Nikon HCA microscopy system, segmented with Cell Profiler, normalized using RUV and lowess using the spatial residuals as controls, and analyzed to identify microenvironment conditions that altered phenotypes of interest.

2016-11-10 | LDS-1469 | LINCS

MEMA HMEC122L Staining Set C

Project description: Not available

2016-11-10 | LDS-1463 | LINCS

LINCS MCF 10A Common Project: Highly-multiplexed imaging by Cyclic Immunofluorescence (CycIF) of protein levels and protein phosphorylation states in the MCF 10A breast cell line treated with 8 small molecule perturbagens. Dataset 1 of 6: Cytosolic fluorescent intensity measurements at 24 hr.

Project description: Not available

2017-05-12 | LDS-1402 | LINCS

LINCS MCF 10A Common Project: Fixed-time-point sensitivity measures of the MCF 10A breast cell line to 8 small molecule perturbagens. Dataset 9 of 9: Calculated dose-response metrics for biological replicate 3

Project description: Not available

2017-03-14 | LDS-1381 | LINCS