Chemoproteomic Profiling Maps Zinc-Dependent Cysteine Reactivity.

ABSTRACT: As a vital micronutrient, zinc is integral to the structure, function, and signaling networks of diverse proteins. Dysregulated zinc levels, due to either excess intake or deficiency, are associated with a spectrum of health disorders. In this context, understanding zinc-regulated biological processes at the molecular level holds significant relevance to public health and clinical practice. Identifying and characterizing zinc-regulated proteins in their diverse proteoforms, however, remain a difficult task in advancing zinc biology. Herein, we address this challenge by developing a quantitative chemical proteomics platform that globally profiles the reactivities of proteinaceous cysteines upon cellular zinc depletion. Exploiting a protein-conjugated resin for the selective removal of Zn

SUBMITTER: Qiu N 

PROVIDER: S-EPMC11957875 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature