Novel tools for genomic modification and heterologous gene expression in the phylum Planctomycetota.

ABSTRACT: Members of the phylum Planctomycetota possess a plethora of intriguing and hitherto underexplored features including an enlarged periplasmic space, asymmetric cell division ("budding"), and a mostly undiscovered small molecule portfolio. Due to the large phylogenetic distance to frequently used and easily genetically accessible model bacteria, most of the established genetic tools are not readily applicable for the here-investigated bacterial phylum. However, techniques for targeted gene inactivation and the introduction of heterologous genes are crucial to investigate the cell biology in the phylum in greater detail. In this study, the targeted genomic modification of model planctomycetes was achieved by enforcing two types of homologous recombination events: simultaneous double homologou

SUBMITTER: Haufschild T 

PROVIDER: S-EPMC11958385 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature