Repair-associated macrophages increase after early-phase microglia attenuation to promote ischemic stroke recovery.

ABSTRACT: Ischemic stroke recovery involves dynamic interactions between the central nervous system and infiltrating immune cells. Peripheral immune cells compete with resident microglia for spatial niches in the brain, but how modulating this balance affects recovery remains unclear. Here, we use PLX5622 to create spatial niches for peripheral immune cells, altering the competition between infiltrating immune cells and resident microglia in male mice following transient middle cerebral artery occlusion (tMCAO). We find that early-phase microglia attenuation promotes long-term functional recovery. This intervention amplifies a subset of monocyte-derived macrophages (RAMf) with reparative properties, characterized by high expression of GPNMB and CD63, enhanced lipid metabolism, and pro-angiogenic act

SUBMITTER: Zhang X 

PROVIDER: S-EPMC11958652 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature