Investigation of new autoantibodies in urothelial bladder cancer for biomarker discovery using immunoproteomics.

ABSTRACT:

Background

Tumor-associated antigens (TAAs) lead to the production of tumor-specific autoantibodies (anti-TAA autoantibodies) by triggering the humoral immune system which can be used as candidate biomarkers. This study aims to investigate TAAs proteins eliciting humoral responses in different stages of urothelial bladder carcinoma (UBC) using an immunoproteomics approach to find novel biomarkers for the clinical management of the disease.

Methods

Total proteins were obtained from the newly established UBC cell line, JAM-ICR, and separated by two-dimensional gel electrophoresis (2DE). These proteins were then immobilized using pooled serum samples from healthy individuals, autoimmune and UBC patients at different stages. The immunoreactive spots in UBC patient samples were i

SUBMITTER: Tabaei S 

PROVIDER: S-EPMC11958914 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature