Hesperidin alleviates pulmonary fibrosis by regulating EI24-mediated autophagy.

ABSTRACT:

Background

Etoposide-induced protein 2.4 (EI24), an essential component of autophagy, is lowly expressed in pulmonary fibrosis. Hesperidin (Hes), a flavonoid, can regulate autophagy in various diseases. However, whether Hes can inhibit pulmonary fibrosis by mechanically regulating EI24-mediated autophagy has not been uncovered.

Methods

RLE-6TN cells were treated with transforming growth factor β1 (TGF-β1) and rats were injected with bleomycin (BLM) to construct the pulmonary fibrosis model. The effect of Hes on pulmonary fibrosis was evaluated by cell counting kit-8, immunofluorescence, hematoxylin and eosin, masson trichome staining and western blotting.

Results

Hes reduced cell viability of TGF-β1-induced RLE-6TN cells. Administration of Hes restored the decrease in

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC11959899 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature