Extrachromosomal circular DNA as a novel biomarker for the progression of colorectal cancer.

ABSTRACT:

Background

Extrachromosomal circular DNA (eccDNA) has potential in tumor diagnosis, particularly for improving diagnostic accuracy and early cancer detection; however, many challenges remain in its application to clinical practice.

Methods

We conducted a Circle-Seq analysis on clinical samples at different stages of colorectal cancer progression to examine the dynamic changes of eccDNA during the progression of colorectal cancer. We used breakpoint-specific PCR to verify candidate eccDNAs identified by Circle-Seq. The results were further validated using the AOM/DSS-induced colorectal cancer model.

Results

There was an increase in the abundance of eccDNA with the progression of colorectal cancer. The genes associated with these eccDNA molecules were primarily related

SUBMITTER: Qiu Q 

PROVIDER: S-EPMC11960012 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature