Co-existence of plasmid-mediated <i>bla</i>_NDM-1 and <i>bla</i>_NDM-5 in <i>Escherichia coli</i> sequence type 167 and ST101 and their discrimination through restriction digestion.

ABSTRACT: The concurrent presence of multiple New Delhi metallo-β-lactamase (bla_NDM) variants within an isolate often goes undetected without next-generation sequencing. This study detects and characterizes dual bla_NDM variants in Escherichia coli through Sanger and whole-genome sequencing. Additionally, a rapid identification method utilizing restriction digestion was designed for detecting bla_NDM variants carrying M154L mutation. Antibiotic susceptibility, minimal inhibitory concentration for meropenem and ertapenem, PCR, and Sanger sequencing of bla_NDM along with genome sequencing using Illumina and Nanopore technology were conducted. Transmissibility and replicon types of bla_NDM-harboring plasmids we

SUBMITTER: Bhattacharjee A 

PROVIDER: S-EPMC11960126 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature