Simplifying SARS-CoV-2 wastewater-based surveillance using an automated FDA EUA assay.

ABSTRACT: Wastewater-based surveillance (WBS) can track the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in communities. Laboratory methods for this testing involve labor-intensive, multi-step processes. This study assessed the feasibility of performing WBS with an off-label use of an automated commercial SARS-CoV-2 assay that had received Emergency Use Authorization for human diagnostic testing from the United States Food and Drug Administration (FDA EUA). Twenty-four-hour composite samples of primary influent wastewater from seven municipalities in New Hampshire and Vermont were collected between September 2020 and February 2021, and were centrifuged upon receipt. An aliquot of fresh supernatant was immediately tested with the Abbott m2000 RealTime SARS-CoV-

SUBMITTER: Sathyanarayana SH 

PROVIDER: S-EPMC11960137 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature