Staphylococcus aureus develops an alternative, ica-independent biofilm in the absence of the arlRS two-component system.

ABSTRACT: The biofilm formation capacity of Staphylococcus aureus clinical isolates is considered an important virulence factor for the establishment of chronic infections. Environmental conditions affect the biofilm formation capacity of S. aureus, indicating the existence of positive and negative regulators of the process. The majority of the screening procedures for identifying genes involved in biofilm development have been focused on genes whose presence is essential for the process. In this report, we have used random transposon mutagenesis and systematic disruption of all S. aureus two-component systems to identify negative regulators of S. aureus biofilm development in a chemically defined medium (Hussain-Hastings-White modified medium [HHWm]). The results of both approaches coincided in tha

SUBMITTER: Toledo-Arana A 

PROVIDER: S-EPMC1196035 | biostudies-literature | 2005 Aug

REPOSITORIES: biostudies-literature