Clonal lineage tracing and transcriptomics of cortical progenitor populations reveal maintenance of differentiation potential.

ABSTRACT: Postnatal neocortical development is a complex period wherein radial glial progenitors (RGPs) complete excitatory neurogenesis and transition to the production of glia. Here, we take advantage of a multi-layered lineage tracing tool pbacBarcode, to examine the contributions of individual cortical RGPs to the postnatal cortex. We reveal that some individual cortical RGPs are multipotent and give rise to olfactory bulb interneurons, astrocytes, and oligodendrocytes in a ∼2:1:1 ratio. We provide evidence that differentiation potential into terminal cell types is maintained as late as post-natal day (P)4, suggesting that a population decline model, as opposed to cell fate restriction, underlies postnatal neocortical development. Moreover, a pool of proliferative intermediary cells, which may r

SUBMITTER: Harkins D 

PROVIDER: S-EPMC11960535 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature