MdfA is a novel ClpC adaptor protein that functions in the developing <i>Bacillus subtilis</i> spore.

ABSTRACT: Bacterial protein degradation machinery consists of chaperone-protease complexes that play vital roles in bacterial growth and development and have sparked interest as novel antimicrobial targets. ClpC-ClpP (ClpCP) is one such chaperone-protease complex, recruited by adaptors to specific functions in the model bacterium Bacillus subtilis and other Gram-positive bacteria, including the pathogens Staphylococcus aureus and Mycobacterium tuberculosis Here we have identified a new ClpCP adaptor protein, MdfA (metabolic differentiation factor A; formerly YjbA), in a genetic screen for factors that help drive B. subtilis toward metabolic dormancy during spore formation. A knockout of mdfA stimulates gene expression in the developing spore, while aberrant express

SUBMITTER: Massoni SC 

PROVIDER: S-EPMC11960690 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature