Investigating drug-liposome interactions using liposomal electrokinetic chromatography.

ABSTRACT: This study explores the potential of using liposomal electrokinetic chromatography as a ranking method for the rapid and simultaneous evaluation of drug-membrane interactions of a larger group of substances and assessing their sensitivity to tissue-specific parameters, namely pH, temperature, and lipid composition. We used a group of nine model drug substances to manifest how molecules could be classified for the relative sensitivity of drug-membrane interactions to pH and temperature. We observed that increasing the amount of liposomes in the background electrolyte significantly affected the separation kinetics of various active pharmaceutical ingredients, altering their mobility and/or peak shapes. Experiments with liposomes from bovine liver and heart tissue extracts revealed different

SUBMITTER: Simonova A 

PROVIDER: S-EPMC11961511 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature