Synergistic and antagonistic activities of IRF8 and FOS enhancer pairs during an immune-cell fate switch.

ABSTRACT: Cell fate instructive genes tend to be regulated by large clusters of enhancers. Whether and how individual enhancers within such clusters cooperate in regulating gene expression is poorly understood. We have previously developed a computational method, SEGCOND, which identifies hubs that we termed Putative Transcriptional Condensates (PTCs), consisting of enhancer clusters and associated target genes. Here, we use SEGCOND to identify PTCs in a CEBPA-induced B-cell-to-macrophage transdifferentiation system. We find that PTCs are enriched for highly expressed, lineage-restricted genes and associate with BRD4, a component of transcriptional condensates. Further, we performed single and combinatorial deletions of enhancers within two PTCs active during induced transdifferentiation, harboring

SUBMITTER: Klonizakis A 

PROVIDER: S-EPMC11961672 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature