The structure of basal body inner junctions from Tetrahymena revealed by electron cryo-tomography.

ABSTRACT: The cilium is a microtubule-based eukaryotic organelle critical for many cellular functions. Its assembly initiates at a basal body and continues as an axoneme that projects out of the cell to form a functional cilium. This assembly process is tightly regulated. However, our knowledge of the molecular architecture and the mechanism of assembly is limited. By applying cryo-electron tomography, we obtained structures of the inner junction in three regions of the cilium from Tetrahymena: the proximal, the central core of the basal body, and the axoneme. We identified several protein components in the basal body. While a few proteins are distributed throughout the entire length of the organelle, many are restricted to specific regions, forming intricate local interaction networks in the inner

SUBMITTER: Li S 

PROVIDER: S-EPMC11961760 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature