A Single-Plasmid Inducible-Replication System for High-Yield Production of Short Ff (f1, M13 or fd)-Phage-Derived Nanorods.

ABSTRACT: Ff (f1, M13 or fd) filamentous phages have been used for myriad applications including phage display, assembly of nanostructures and as carriers of agents used for diagnostic and therapeutic purposes. Recently, short Ff phage-derived functionalised nanorods have emerged as a superior alternative to full-length filamentous phages for applications from lateral flow assays to cell- and tissue-targeting. Their advantages, such as shorter length and the lack of antibiotic resistance genes, make them particularly promising for expanding the current scope of Ff bionanotechnology and biomedical applications. Limitations to the widespread use of Ff-derived nanorods include a requirement for two plasmids and the relatively low production efficiency. This is due to the presence of only the positive F

SUBMITTER: Leon-Quezada RI 

PROVIDER: S-EPMC11962056 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature