Rapid detection of <i>Mycoplasma pneumoniae</i> CARDS toxin in clinical respiratory specimens by a loop-mediated isothermal amplification assay.

ABSTRACT: In light of the absence of rapid and precise diagnostic laboratory tests for the detection of Mycoplasma pneumoniae (MP), a prominent etiological agent implicated in a range of respiratory infections, we developed and evaluated a rapid and straightforward loop-mediated isothermal amplification (LAMP) assay targeting the MP community-acquired respiratory distress syndrome toxin (CARDS TX) gene. The LAMP assay was performed at 65°C for a duration of 60 min, yielding a minimum detection concentration of MP CARDS TX at 0.4986 pg/μl. The assay exhibited no cross-reactivity with 13 other prevalent pathogens associated with respiratory infections or with other common bacterial toxin genes. To further substantiate the validity of the LAMP assay, 200 pharyngeal swabs or bronchoalveolar lavag

SUBMITTER: Fang Y 

PROVIDER: S-EPMC11962300 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature