Flycodes enable simultaneous preclinical analysis for dozens of antibodies in single cassette-dosed mice.

ABSTRACT: Protein therapeutics such as antibodies require in-depth in vivo characterization during development and consequently account for a large proportion of laboratory animal consumption in the pharmaceutical industry. Currently, antibody candidates are exhaustively tested one-by-one in animal models to determine pharmacokinetic and pharmacodynamic (PK/PD) profiles. The simultaneous analysis of antibody mixtures in single animals, called cassette-dosing, could in principle overcome this bottleneck, but is currently limited to small cassette sizes. Here, we demonstrate how the use of genetically encoded peptide tags (flycodes), designed for maximal detectability in liquid chromatography-mass spectrometry, can allow for the simultaneous characterization of large pools of drug candidates, from sin

SUBMITTER: Walter JD 

PROVIDER: S-EPMC11962451 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature