Highly spatial-temporal electrochemical profiling of molecules trafficking at a single mitochondrion in one living cell.

ABSTRACT: Simultaneous profiling of multiple molecules trafficking at a single organelle and the surrounding cytosol within a living cell is crucial for elucidating their functions, necessitating advanced techniques that provide high spatial-temporal resolution and molecule specificity. In this study, we present an electrochemical nanodevice based on a θ-nanopipette designed to coanalyze calcium ions (Ca²⁺) and reactive oxygen species (ROS) at a single mitochondrion and its surrounding cytosol, thereby enhancing our understanding of their trafficking within the signaling pathways of cellular autophagy. Two independent nanosensors integrated within the channels of the θ-nanopipette spatially isolate a single target mitochondrion from the cytosol and simultaneously measure the release of Ca

SUBMITTER: Liu K 

PROVIDER: S-EPMC11962482 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature