Exoproteomic Evidence for BcTSPO-Mediated Regulation of Virulence Factors in Bacillus cereus.

ABSTRACT: Bacillus cereus translocator protein (BcTSPO) is a transmembrane protein that plays a regulatory role in various Bacillus cereus phenotypes, potentially including its virulence. Given that the exoproteome is a major determinant of B. cereus virulence, particularly during the late stages of active growth, its analysis is crucial for understanding the regulatory functions of BcTSPO. In this study, we conducted a comparative analysis of the exoproteomes and cellular proteomes of a Δtspo mutant and the wild-type ATCC 14579 strain during the late exponential and early stationary growth phases. Shotgun proteomics revealed that the absence of BcTSPO significantly depleted the exoproteome of secreted virulence factors, including the HBL, NHE, and CytK enterotoxins. Interestingly, the cholesterol-d

SUBMITTER: Duport C 

PROVIDER: S-EPMC11962584 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature