PAGE-based transfer learning from single-cell to bulk sequencing enhances model generalization for sepsis diagnosis.

ABSTRACT: Sepsis, caused by infections, sparks a dangerous bodily response. The transcriptional expression patterns of host responses aid in the diagnosis of sepsis, but the challenge lies in their limited generalization capabilities. To facilitate sepsis diagnosis, we present an updated version of single-cell Pair-wise Analysis of Gene Expression (scPAGE) using transfer learning method, scPAGE2, dedicated to data fusion between single-cell and bulk transcriptome. Compared to scPAGE, the upgrade to scPAGE2 featured ameliorated Differentially Expressed Gene Pairs (DEPs) for pretraining a model in single-cell transcriptome and retrained it using bulk transcriptome data to construct a sepsis diagnostic model, which effectively transferred cell-layer information from single-cell to bulk transcriptome. S

SUBMITTER: Jin N 

PROVIDER: S-EPMC11962595 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature