A comprehensive strategy for the development of a multi-epitope vaccine targeting <i>Treponema pallidum</i>, utilizing heat shock proteins, encompassing the entire process from vaccine design to <i>in vitro</i> evaluation of immunogenicity.

ABSTRACT:

Background

Treponema pallidum, the causative spirochete of syphilis, is primarily transmitted through sexual contact and has emerged as a significant global health concern. To address this issue, enhancing diagnostic capabilities, strengthening public health interventions, and developing a safe and effective vaccine are critical strategies.

Objective

This study employed an immunoinformatics approach to design a vaccine with high immunogenic potential, targeting the heat shock proteins of T. pallidum.

Methods

Based on heat shock proteins of T. pallidum, we predicted B-cell, CTL, and HTL epitopes and all the selected epitopes were linked to construct a multi-epitope vaccine. Antigenicity, toxicity, and allergenicity of epitopes were checked by VaxiJ

SUBMITTER: Jiang J 

PROVIDER: S-EPMC11962626 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature