Targeting PD-1 and CD85j can restore intratumoral CD4⁺ GzmB⁺ T-cell functions to combat MHC-II-expressing tumors.

ABSTRACT:

Background

A subset of CD4⁺ T cells with cytotoxic activity has been identified, and these cells exert their effects by expressing perforin and granzymes. Despite the progress made in characterizing cytotoxic CD4⁺ T cells in various diseases, the status of cytotoxic CD4⁺ T cells in non-small cell lung cancer (NSCLC) and the underlying mechanisms involved in promoting intratumoral cytotoxic CD4⁺ T-cell activation remain unclear.

Methods

We used flow cytometry to examine the phenotypic and functional properties of CD4⁺GzmB⁺ T cells in the peripheral blood and tumor tissues of patients with NSCLC. Loss-of-function analyses and RNA sequencing were used to identify the underlying mechanisms involved in the effects

SUBMITTER: Wang B 

PROVIDER: S-EPMC11962805 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature