Deletion of the <i>chloroquine resistance transporter</i> gene confers reduced piperaquine susceptibility to the rodent malaria parasite <i>Plasmodium berghei</i>.

ABSTRACT: Malaria parasites acquire drug resistance through genetic changes, the mechanisms of which remain incompletely understood. Understanding the mechanisms of drug resistance is crucial for the development of effective treatments against malaria, and for this purpose, new genetic tools are needed. In a previous study, as a forward genetic tool, we developed the rodent malaria parasite Plasmodium berghei mutator (PbMut) line, which has a greatly increased rate of mutation accumulation and from which we isolated a mutant with reduced susceptibility to piperaquine (PPQ). We identified a mutation in the chloroquine resistance transporter (PbCRT N331I) as responsible for this phenotype. In the current study, we generated a marker-free PbMut to enable further genetic manipulation of th

SUBMITTER: Hirai M 

PROVIDER: S-EPMC11963562 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature