Chromosomal genes modulating fosfomycin susceptibility in uropathogenic <i>Escherichia coli</i>: a genome-wide analysis.

ABSTRACT: Escherichia coli acquires fosfomycin resistance through chromosomal mutations that reduce drug uptake and by drug-inactivating enzymes. However, the complete resistance mechanisms remain to be elucidated. The aim of this study was to elucidate the genetic mechanisms regulating fosfomycin susceptibility in uropathogenic E. coli (UPEC). We constructed a highly saturated transposon mutant library containing >340,000 unique Tn5 insertions in a clinical UPEC strain. We conducted transposon-directed insertion site sequencing (TraDIS) to screen for chromosomal genes whose mutations are beneficial for bacterial growth and survival in the presence of fosfomycin at 4 and 32 µg/mL. TraDIS analysis identified 67 genes including known resistance determinants (n = 13) as well as a s

SUBMITTER: Ma Y 

PROVIDER: S-EPMC11963563 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature