A method for in-depth analysis of circular DNA virus populations by unambiguously profiling the low abundant virus variants and partial genomic components.

ABSTRACT: Severe epidemic outbreaks of diseases associated with newly emerging strains of single-stranded DNA (ssDNA) viruses have led to serious economic losses of numerous important food crops. While the current mitigation strategies are mostly relying on the deployment of genetic resistance in crop varieties, the constantly evolving virus populations have the potential to rapidly break virus resistance. Therefore, the development of diagnostic tools enabling early detection of virus variants associated with hypervirulence and/or expansion to new host species is urgently needed as an effective mitigation solution. Here, we introduce a novel approach by designing a pipeline that allows accurately identifying and characterizing the full-length sequence variants of viral circular DNA genomes utilizin

SUBMITTER: Golyaev V 

PROVIDER: S-EPMC11963754 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature