Comparison of histochemical methods for the analysis of eosinophils and mast cells using a porcine model of eosinophilic esophagitis.

ABSTRACT:

Introduction

Accurate identification of eosinophils in tissue sections is required for diagnosis of eosinophilic esophagitis in humans and the assessment of severity of disease in allergy models. The pig may be a good model for sensitization and allergy models due to anatomical, physiological, and immunological similarities to humans. However, comparative studies on histochemical detection of eosinophils in fixed porcine tissue are lacking.

Methods

Qualitative and quantitative comparisons were performed for six histochemical methods previously reported for eosinophil and mast cell detection in other species. Astra Blue/Vital New Red, Congo Red, Luna, Sirius Red, Toluidine Blue, and modified regressive Hematoxylin & Eosin were applied to formalin-fixed paraffin embedded full-

SUBMITTER: Snider DB 

PROVIDER: S-EPMC11963769 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature