High-Throughput Culture and DNA Isolation Methods for Aspergillus fumigatus.

ABSTRACT: Aspergillus fumigatus is an opportunistic human fungal pathogen that is widely spread in the environment. The need to screen a large number of environmental or clinical isolates for phenotypic and/or genotypic purposes can be challenging and time consuming when using the protocols that are currently available. We have developed a novel approach that allows one to inoculate cultures, grow individual A. fumigatus isolates, and subsequently isolate DNA of sufficient quality for PCR and/or whole genome sequencing, all in a 96-well format. Compared to currently available methods, our protocols reduce both cost and labor significantly and are compatible with any experimental setup that uses a 96-well format. © 2025 The Author(s). Current Protocols published by Wiley Periodicals LLC. Basic Protoc

SUBMITTER: Reyes Marquez FC 

PROVIDER: S-EPMC11963831 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature