Obtaining complete and canonical ammonia-oxidizing bacteria through specific labeling and cell sorting.

ABSTRACT: Mitigation of the negative environmental consequences of excess anthropogenic nitrogen input requires a thorough understanding of the processes driving the biogeochemical nitrogen cycle. Nitrification is one of the key nitrogen-cycling processes and is performed by ammonia-oxidizing bacteria and archaea, nitrite-oxidizing bacteria, and complete nitrifiers. However, the fastidious growth of nitrifiers largely hampered their isolation using classical cultivation techniques, as most nitrifiers do not grow on solid media. Here, we present a workflow for the targeted enrichment and isolation of complete and canonical ammonia-oxidizing bacteria by combining function-specific in vivo fluorescent labeling with cell sorting. Optimized floc disruption and labeling techniques enlarged the fraction of

SUBMITTER: Blom P 

PROVIDER: S-EPMC11964087 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature