Critical functions and key interactions mediated by the RNase E scaffolding domain in Pseudomonas aeruginosa.

ABSTRACT: The RNA degradosome is a bacterial multi-protein complex mediating mRNA processing and degradation. In Pseudomonadota, this complex assembles on the C-terminal domain (CTD) of RNase E through short linear motifs (SLiMs) that determine its composition and functionality. In the human pathogen Pseudomonas aeruginosa, the RNase E CTD exhibits limited similarity to that of model organisms, impeding our understanding of RNA metabolic processes in this bacterium. Our study systematically maps the interactions mediated by the P. aeruginosa RNase E CTD and highlights its critical role in transcript regulation and cellular functions. We identified the SLiMs crucial for membrane attachment, RNA binding and complex clustering, as well as for direct binding to the core components PNPase and RhlB. Trans

SUBMITTER: Geslain SAM 

PROVIDER: S-EPMC11964227 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature