Molecular characterization of the effects of heat shock on the infection cycle progression and productivity of the baculovirus expression vector system.

ABSTRACT: Baculoviruses are widely utilized in biotechnology for various purposes, including recombinant protein expression, antigen presentation, vaccine production, as biopesticides, and as gene therapy vectors. The productivity of the baculovirus expression vector system (BEVS) is significantly affected by the condition of the host cell. However, the impact of host cell stress on the complex baculovirus infection cycle remains not fully understood. This study examines the effects of three gradual heat shock treatments on the production of recombinant protein and viral titers in Sf9 cells (Spodoptera frugiperda) infected with a recombinant baculovirus AcMNPV with fluorescent reporters under late (vp39) and very late (polh) promoters. The heat shock regimens applied before infection were 30°C for 2

SUBMITTER: Paz-Cortes E 

PROVIDER: S-EPMC11964234 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature