A Cross-Sectional Study on Comparison of Serum Cortisol Concentration Measured by Chemiluminescent Immunoassay in Four Different Automated Analyzers in a Variety of Adrenal Disorders.

ABSTRACT:

Introduction

Cortisol concentration is often estimated by competitive chemiluminescent immunoassays, which are prone to interference due to cross-reactivity. The extent of interference is inconsistent across different adrenal disorders and among different analyzers. Although liquid chromatography-mass spectrometry (LC-MS/MS) possesses better analytical specificity, it is not widely available. We aimed to compare cortisol values measured across four different analyzers across different adrenal disorders.

Methods

Cortisol concentrations were measured in the serum of patients with congenital adrenal hyperplasia (CAH) (n = 12), primary adrenal insufficiency (PAI) (n = 11), endogenous Cushing's syndrome (CS) (n = 6), adrenal incidentaloma (AI) (n = 8),

SUBMITTER: Agrawal N 

PROVIDER: S-EPMC11964372 | biostudies-literature | 2025 Jan-Feb

REPOSITORIES: biostudies-literature