Cloning, phylogenetic analysis, tissue expression profiling, and functional roles of NPC1L1 in chickens, quails, and ducks.

ABSTRACT: The Niemann-Pick C1-Like 1 (NPC1L1) protein, primarily expressed in the epithelial cells of the small intestine, is essential for cholesterol absorption from both dietary intake and biliary secretion. Despite this conserved function across mammals, the full-length coding sequence of NPC1L1 remains uncharacterized in key avian models including chicken (Gallus gallus), quail (Coturnix japonica), and duck (Anas platyrhynchos). In this study, we successfully cloned the full NPC1L1 mRNA sequence in chicken, quail, and duck, including the entire 5' and 3' untranslated regions, utilizing rapid amplification of cDNA ends methods. Phylogenetic analysis across 12 species, comprising four avian and eight representative mammalian species, revealed that the NPC1L1 sequences in the main poultry species

SUBMITTER: Shu X 

PROVIDER: S-EPMC11964625 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature