Pre-clinical model of dysregulated FicD AMPylation causes diabetes by disrupting pancreatic endocrine homeostasis.

ABSTRACT: The bi-functional enzyme FicD catalyzes AMPylation and deAMPylation of the endoplasmic reticulum chaperone BiP to modulate ER homeostasis and the unfolded protein response (UPR). Human hFicD with an arginine-to-serine mutation disrupts FicD deAMPylation activity resulting in severe neonatal diabetes. We generated the mFicD^R371S mutation in mice to create a pre-clinical murine model for neonatal diabetes. We observed elevated BiP AMPylation levels across multiple tissues and signature markers for diabetes including glucose intolerance and reduced serum insulin levels. While the pancreas of mFicD^R371S mice appeared normal at birth, adult mFicD^R371S mice displayed disturbed pancreatic islet organization that progressed with age. mFicD^R371S mice prov

SUBMITTER: Casey AK 

PROVIDER: S-EPMC11964657 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature